Purification of the recombinant green fluorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography

Table 1 Quantitative specifications of the principal stages of GFP purification

Processing step	TSP^a (mg)	GFP^b (mg)	Purity^c (%)	GFP Yield ^d (%)
Extract	13.7	8.2	59.9	100.0
Ethanol extraction	5.5	4.8	87.3	58.5
n-butanol extraction	4.7	4.2	89.4	51.2
HiScreen Capto Butyl	2.9	2.8	96.6	34.1

^aAmount of the TSP was determined according to BCA
^bAmount of the GFP was measured by the ELISA
^cPurity is defined as the amount of GFP divided by the amount of TSP in the same sample
^dGFP Yield is defined as the amount of GFP recovered divided by initial amount of GFP in the crude extract of inoculated leaves

ISSN: 1472-6750