Table 3 Primers used in this study
Primers | Sequences | RE |
|---|---|---|
F-attB | CTACTGCTGCTTCACCAGTTT | – |
R-attB | GTATGCAGCGATGTTGTTACCC | – |
F-attP | GGAC CTCGAGTCCAACTCGCTTAATTGC | XhoI |
R-attP | GGACGGATCCGCTAAAACGTCTCAGAAA | BamHI |
F-Int | GACGCCATGGCACATCATCATCATCATCATATGACTAAGAAAGTAGCAATCTATAC | NcoI |
R-Int | GCACGAGCTCTTAAGCGAGTTGGAATTTAAATATG | HindIII |
F-PnisA | GCGAGATCTAGTCTTATAACTATAC | BglII |
R-PnisA | GCAGATATCGTGAGTGCCTCCTTATAAT | EcoRV |
F-P170 | GCGACAGATCTGAACTATGAATATC | BglII |
R-P170 | GCACGATATCAACTGTTCTTTTTTAATTTTT | EcoRV |
F-USP45 | GACGGATATCATGAAAAAAAAGA | EcoRV |
R-USP45 | CTGAGTCGACGATATTTCGAGAGC | SalI |
F-SpkNuc | GCGTGATATCATGAAAAAAATATTAACGTTGGTAT | EcoRV |
R-NucH | GGCGAGCTCTTAGTGGTGATGATGGTGA | SacI |
F-NucEV | GCGTGATATCATGAAAAAAATATTAACGTTGGTAT | EcoRV |
F-NucNI | CGAGCCATGGCTATGAAAAAAATATTAACG | NcoI |
R-NucEI | CGCGGAATTCTTGACCTGAATCAGCGTTGTCTTCG | EcoRI |
F-Em | GACGGGATCCAATCAGGCTTGATCCCCAGTAAGTC | BamHI |
R-Em | GACGAGATCTAGGATGAGGAGGCAGATTGCCTTG | BglII |
F-PrtP344 | GAGCGAATTCTGGCTCTAGAGGATCCAAGTC | EcoRI |
R-PrtP344 | TAAACTATGCGGCCGCGACAGGCTATTCTTC | NotI |
F-E. coli Ori | GGAGGGATCCGGCGTAATCATGGTCATAG | BamHI |
R-E. coli Ori | AGGCGGATCCCTGTCAGACCAAGTTTACTC | BamHI |