Skip to main content

Table 1 Single targeting of TRP53-R172P mice with CRISPR/Cas9 system

From: Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects

Cas9/gRNA/donor (ng/μl)

Survival/ Injected zygotes

Transferred embryos/ Recipients

Newborns

40/17.5/60

167/210

156/6

26

Total mice

WT

KO (double peaks)

KI

26

15/26

11/26

3/26

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us