Table 3 Primers used in the study
Name | No. | Sequence (5′ → 3′) | Annotation |
|---|---|---|---|
mtpA up-fw-U | 1 | GGGTTTAAUAGACATACCGTCGACCTTGG | Amplify upstream region of mtpA |
mtpA up-rv-U | 2 | GGACTTAAUGAGGGTGAGTCTGGCAGAAG | Amplify upstream region of mtpA |
mtpA do-fw-U | 3 | GGCATTAAUTCAGTTTTGCATGGTTGAGC | Amplify downstream region of mtpA |
mtpA do-rv-U | 4 | GGTCTTAAUGCGGGTGGTATTCTCTGTGT | Amplify downstream region of mtpA |
Bipart-1 rv | 5 | GATGTTGGCGACCTCGTATT | Amplify 1st bipartite fragment |
Bipart-2 fw | 6 | GATGTAGGAGGGCGTGGATA | Amplify 2nd bipartite fragment |
mtpA up-fw | 7 | AGACATACCGTCGACCTTGG | Amplify 1st bipartite fragment |
mtpA do-rv | 8 | GCGGGTGGTATTCTCTGTGT | Amplify 2nd bipartite fragment |
mtpA ko-ch-fw | 9 | GTCGCAAGCTTCAACTTTCC | Check for positive knockout of mtpA |
mtpA ko-ch-rv | 10 | TATTGGAGAGCAAGGGATGG | Check for positive knockout of mtpA |
Hph-Fw | 11 | GATGTAGGAGGGCGTGGATA | Amplify hph marker |
Hph-Rv | 12 | GATGTTGGCGACCTCGTATT | Amplify hph marker |
mtpA-fw | 13 | CAAGTTCTCCTTGAGTGAGTCG | Transcription analysis of mtpA |
mtpA-rv | 14 | TCGACTGCCTTTACAAGACC | Transcription analysis of mtpA |