Fig. 6

Binding of the divalent scFvF7-Fc Ab on antigen-specific CD8⁺ T lymphocytes. Both Nef-and Mart1-specific human CD8⁺ T lymphocytes were stimulated through co-cultivation with HLA-matched B-LCLs previously treated with either specific (stimulated) or mismatched (unstimulated) peptides. After o.n. co-cultivation, the co-cultures were labelled with the reconstituted F7 Ab, followed by incubation with FITC-conjugated anti-human IgGs. Then, cells were labeled with PE-conjugated anti-CD8 mAb. As control, PBMC-derived CD8⁺ T cells from either unstimulated or PHA-activated were labelled with either IgGs or the divalent scFvF7-Fc Ab (upper panels). In the bottom panels, histograms of FITC-related fluorescence profiles of PE-positive cells are shown. Results from a representative of four independent experiments are reported