Table 1 Oligonucleotide primer pairs used for the construction of mutant enzymes
From: Structure-based engineering of heparinase I with improved specific activity for degrading heparin
S. no. | Amino acid change | Primer sequencesa |
|---|---|---|
1 | S169D | 5′-CAGTGGCACGGTGCTCCGGAT(GCT)CGTACTCTTGTTGCTACT − 3′ 5′-AGTAGCAACAAGAGTACGATC(AGC)CGGAGCACCGTGCCACTG − 3′ |
2 | A259D | 5′-GTGGCTTACTGATAAAGAT(GAA)GATCGTAACAACGCTA-3′ 5′-TAGCGTTGTTACGATCATC(TTC)TTTATCAGTAAGCCAC-3′ |