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Table 2 Calculated apparent kinetic parameters of each mutant compared to wt NicA2

From: Optimization of a nicotine degrading enzyme for potential use in treatment of nicotine addiction

Variant kcat/Km (M−1 s−1)
(apparent)
kcat (s−1)
(apparent)
Km (nM)
(apparent)
wt NicA2 6.4 × 104 0.015 240
A107R 1.9 × 105 0.159 830
A107T 1.8 × 105 0.050 285
A107G 1.6 × 105 0.053 335
F104 T 1.7 × 105 0.055 330
F104I 1.6 × 105 0.039 240
F104R 1.5 × 105 0.041 270
F104S 1.3 × 105 0.031 250
G106A 1.1 × 105 0.031 280
F355H 1.3 × 105 0.029 220
A426C 1.3 × 105 0.030 230
  1. Apparent kcat/Km values were calculated using the low nicotine assay results (Fig. 2c) using a form of the Michaelis-Menten equation (v0/[E] = kcat/Km*[S]) and to construct progress curves that were fitted to a simple kinetic model (KinTek) to derive apparent kcat values. The resulting kcat/Km and kcat values were used to calculate the apparent Km values