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Table 1 Sequence information for artificial 35Sp LAMP templates

From: Lack of specificity associated with using molecular beacons in loop mediated amplification assays

Target, Notation, Version

Template Sequence (5’ to 3’)

35Sp BIP Dumbbell, LOOPS, PH

CACGTCTTCAAAGCAAGTGGGGATAGTGGGAT

 

TGTGCGTCCCCTTACGTCAGTGGACCACTTGC

 

TTTGAAGACGTGGTCTAGATTCCACGATGCTC

 

CTCGGGGGTCCATCTTTGGGCCACTGTCGGCA

 

GAGGCGAGGAGCATCGTGGAA

35Sp partial sequence, LINEAR, PH

TTGCGATAAAGGAAAGGCCATCGTTGAAGATG

 

CCTCTGCCGACAGTGGTCCCAAAGATGGACCC

 

CCACCCACGAGGAGCATCGTGGAAAAAGAAGA

 

CGTTCCAACCACGTCTTCAAAGCAAGTGGATT

 

GATGTGATATCTCCACTGACGT

  1. 35Sp templates synthesised and PAGE purified by IDT (Coralville, Iowa, USA). Both oligonucleotides are 150 bases in length, diluted to 10 micromolar and stored at minus 20 degrees C
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BMC Biotechnology

ISSN: 1472-6750

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