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Fig. 4 | BMC Biotechnology

Fig. 4

From: Lack of specificity associated with using molecular beacons in loop mediated amplification assays

Fig. 4

Lack of enhanced specificity with LAMP molecular beacons. 1A denotes 35S promoter LAMP amplification of an optimised 35Sp template with amplification detection using SYTO9 dye (positive template control in blue and NTC in orange), 1B shows detection of 35Sp LAMP amplification with 35Sp 5nt MB and 1C shows detection of 35Sp LAMP amplification with NOSt 5t MB. 2A denotes LTR LAMP amplification of a plasmid using SYTO9 dye, 2B shows detection of the LTR LAMP amplification with 35Sp LoopF MB, 2C shows LTR LAMP with NOSt 5nt STEM molecular beacon detection, 2D shows LTR LAMP with 35Sp 4nt STEM MB, 2E with 35Sp 5nt STEM MB and 2F with 35Sp 6nt STEM MB

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