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Fig. 11 | BMC Biotechnology

Fig. 11

From: Lack of specificity associated with using molecular beacons in loop mediated amplification assays

Fig. 11

LAMP MBs with PCR and LAMP amplification 1 and 2 represent LAMP assays whereas 3 and 4 are PCR results. 1 and 3 represent assays with SYTO9 and the 35Sp 5nt MB, 2 and 4 include SYTO9 but no LAMP MB (template results in blue and NTCs in orange). Fluorescent detection of SYTO9 are shown in A. Detection of the presence or absence of the 35Sp 5nt JOE MB are shown in B with corresponding melt curves in C. 2D and 4D show the melt curve for the amplified samples from 2 and 4 after the post amplification addition of the LAMP MB

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