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Table 1 High-quality LASSO captures for both target and non-target sequences

From: Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning

  Captured Sequences (> 10-fold Coverage) Expected Sequences
50 μl 2 ml
Targets 590 1282 3164
Non-Targets 454 388 4434
  1. Fold-coverage refers to the number of times that a certain sequence is fully sequenced. High-quality captures were strictly defined as sequences with more than ten-fold sequencing coverage, calculated as a function of sequencing read length (50 bp for HiSeq sequencing platform) multiplied by aligned read depth divided by sequence length