Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning

BMC Biotechnology

Table 1 High-quality LASSO captures for both target and non-target sequences

	Captured Sequences (> 10-fold Coverage)		Expected Sequences
	50 μl	2 ml	Expected Sequences
Targets	590	1282	3164
Non-Targets	454	388	4434

Fold-coverage refers to the number of times that a certain sequence is fully sequenced. High-quality captures were strictly defined as sequences with more than ten-fold sequencing coverage, calculated as a function of sequencing read length (50 bp for HiSeq sequencing platform) multiplied by aligned read depth divided by sequence length

ISSN: 1472-6750