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Table 2 Summarized kinetic parameters of cell growth and natamycin production by S. natalensis NRRL 2651 under different cultivation conditions applied

From: Enhanced Natamycin production by Streptomyces natalensis in shake-flasks and stirred tank bioreactor under batch and fed-batch conditions

Parameter

Cultivation conditions

Shake-flask cultivation

Bioreactor cultivation

Batch (20 g/L)

Intermittent feeding (glucose+beef)

Intermittent feeding (glucose)

Batch (20 g/L)

Glucose feeding

Xmax (g/L)

4.780 ± 0.13

12.25 ± 0.45

9.030 ± 0.84

6.460 ± 0.04

6.21 ± 0.05

Pmax (g/L)

1.580 ± 0.03

1.855 ± 0.1

2.480 ± 0.04

1.710 ± 0.01

2.72 ± 0.01

Qxa (g/L/h)

0.099

0.080

0.085

0.111

0.103b

Qpa (g/L/h)

0.021

0.022

0.023

0.038

0.045b

Qsa (g/L/h)

−0.41

−0.31

−0.220

−0.443

−0.448

Yx/s (g/g)

0.280

0.260

0.280

0.760

0.61

Yp/s (g/g)

0.083

0.083

0.086

0.090

0.162

Yp/x (g/g)

0.332

0.320

0.330

0.280

0.46

  1. Xmax: maximal cell dry weight; Pmax: maximal natamycin production; QX: average cell growth rate; QP: average natamycin production rate; QS: average glucose consumption rate; YX/S: maximal yield of biomass per consumed glucose; YP/S: maximal yield of natamycin per consumed glucose; YP/X: maximal yield of natamycin per biomass. a Data are calculated at the end of the exponential growth phase. b Data are taken during the feeding phase