Skip to main content
Fig. 1 | BMC Biotechnology

Fig. 1

From: Improved the expression level of active transglutaminase by directional increasing copy of mtg gene in Pichia pastoris

Fig. 1

Strategy for the construction of mtg and pro co-expression strains. This schematic map represents the constructed expression vectors for the pro and mtg, designated pGAP9/pro and pPICZα-rDNA/mtg, respectively. The his4 and rDNA non-coding sequences allow the vector to be inserted into the corresponding sites in the genome of strain GS115 through homologous recombination. The pPICZα-rDNA-mtg vector was constructed by introducing a non-coding rDNA (indicated as rDNAnc) sequence into the pPICZα vector. The expression of both pro and mtg were under the control of the GAP and AOX1 promoters, respectively

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us