Purification and biochemical characterization of a novel thermostable protease from the oyster mushroom Pleurotus sajor-caju strain CTM10057 with industrial interest

Table 2 Alignment of the NH₂-terminal amino-acid sequence of the purified SPPS from Pleurotus sajor-caju strain CTM10057 with the sequences of other fungal proteases

Enzyme	Origin	NH₂-terminal amino-acid ^a,b	Identity (%)
SPPS (this work)	Pleurotus sajor-caju strain CTM10057	GPEDPALPPDSESTHVITGVEKLHAQ	–
PoSl	Pleurotus ostreatus strain ATCC® MYA-2306™	GP D DPALPPD	90
PA_PoS1	Schizopora paradoxa strain KUC8140	GP T DPA I PPDSESTHV L TGV	85
VPR	Phlebia centrifuga strain FBCC195	DDPAI PPD T ESTHV L TGV D KLHAQ	79
PcSl	Phanerochaete chrysosporium strain S1	G KS DPA V P A D T ESTH	75
PR1C	Metarhizium anisopliae strain ARSEF 549	LSSR A DDGA D IF S P HV MTQV KL	64

^a Amino-acid sequences for comparison were obtained using the program BLASTP (NCBI, NIH, USA) database with default parameters
^b Residues not identical with SPPS protease from Pleurotus sajor-caju strain CTM10057 are indicated in black box

ISSN: 1472-6750