Fig. 5

An α-retrovector mediating preferential transgene expression in β cells. EndoC-βΗ2 and 293 T cells were transduced with the indicated α-retrovectors using TVA-gesicles. Cells were harvested 8 days later and GFP fluorescence was analyzed by flow cytometry. For cells transduced with a selectable construct (GFP-IRES-ZeoR), 8 days corresponds to 3 days without zeocin and 5 days with zeocin (3 + 5). The number above each diagram indicates the mean of fluorescence of the whole population of analyzed cells. The number in the diagram is the percentage of fluorescent cells. This experiment was performed once, with two distinct reporters in the three α-retrovectors giving similar results