Fig. 2

A SIN γ-retrovector mediating preferential transgene expression in β cells. Human β (EndoC-βΗ2) and non-β (293 T) cells were transduced in parallel with the indicated VSV-G pseudotyped γ-retrovectors. Cells were harvested 4 days later (without selection) and the level of GFP or YFP fluorescence was analyzed by flow cytometry. The number above each diagram indicates the mean of fluorescence of the whole population of analyzed cells. The number in the diagram is the percentage of fluorescent cells. This experiment was performed once, the two different pSERS RIP constructs giving similar results. Note that the positive threshold level is slightly higher for 293 T cells transduced with pPRIZ GFP to take into account an increase in the autofluorescence level of negative cells (lower cloud). FSC-A: Forward-Scatter-Area