Fig. 5
From: Investigation of cell culture conditions for optimal foot-and-mouth disease virus production
Cell viability of BHK-2P cells at increasing densities and different media substitutions. A substitution of 30% or 100% fresh media was performed before infection. Cells were infected with FMDV A IRN/8/2015 (a) or O SAU/18/2015 (b). Significant differences in cell viability 20 hpi were detected between 30 and 100% media substitution at cell densities of 2 × 106 cells/mL and 3 × 106 cells/mL for A IRN/8/2015 and between the 30% media exchange preparations of 1 × 106 cells/mL and 3 × 106 cells/mL for O SAU/18/2015. Experiments were performed three times independently. Significance code: *** p < 0.001; **** p < 0.0001