Fig. 4
From: Investigation of cell culture conditions for optimal foot-and-mouth disease virus production
Difference in content of glucose (a), lactate (b) and glutamine (c) in the cell culture media with increasing concentrations of ammonium chloride. BHK-2P cells were maintained in ACF media and infected with either A IRN/8/2015 (open circles) or O SAU/18/2015 (filled squares). Experiments were performed three times independently. The content of glucose was determined in duplicates for every experiment. The content of lactate and glutamine was determined three times individually. No statistically significant differences in the difference of content for glucose,glutamine or lactate could be detected between the conditions in infected cells. Nevertheless, an increase in the contents of glutamine and lactate is evident at ammonium chloride concentrations above 6 mM