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Fig. 6 | BMC Biotechnology

Fig. 6

From: Production of alkaline pectinase: a case study investigating the use of tobacco stalk with the newly isolated strain Bacillus tequilensis CAS-MEI-2-33

Fig. 6

The purification of alkaline pectinase from B. tequilensis CAS-MEI-2-33. a Ammonium sulfate fractionation curve; b Elution curve of Mini Macro-Prep High-Q ion exchange chromatography; c. Elution curve of Sephacryl S-100 column chromatography; d. The partial purification of alkaline pectinase from B. tequilensis CAS-MEI-2-33 using TS. M: molecular weight makers; 1. Sephacryl S-100 column chromatography with pH 7.2; 2: Mini Macro-Prep High-Q ion exchange chromatography with pH 8.0; 3. Concentrated ammonium sulfate salting out solution

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