Frequency of off-targeting in genome edited pigs produced via direct injection of the CRISPR/Cas9 system into developing embryos

Table 2 Summary of off-targeting events in AR gene and RBFOX1 gene. Ten pigs were analyzed for off-target events. Eight potential off-targets occurred in AR gene. Seven potential off-targets occurred in RBFOX1 gene

Ig Heavy chain
Pig	AR gene	WT present?	RBFOX1 gene	WT present?
WT	TCTCCCCCCAGGTTTTTGTGAGG	N/A	ACACCTCCCAGGCTTTTGTGG	N/A
A	TCTCCCCCCAGGTTTTTGTGAGG TCTCCCCCCAGGTTTTTTGTGAGG	yes	ACACCTCCCAGGCTTTTGTGG	yes
B	TCTCCCCCCAGGTTTTTGTGAGG	yes	ACACCTCCCAGGCTTTTGTGG	yes
C	TCTCCCCCCAGGTTTTTGTGAGG TCTCCCCCCAGGTTTTTTTGTGA	yes	ACACCTCCCAGGCTTGCAGGA	no
D	TCTCCCCCCAGGTTTTTGTGAGG TCTCCCCCCAGGTGAGGGAAGA	yes	ACACCTCCCAGGCTTTTGGTG ACACCTCCCAGGCTTGCTGTA	no
E	TCT CCCCCCAGGTTTTTGGTGAGG TCTCCCCCCAGGTTTTTGGGGGG	no	ACACCTCCCAGGCTTAGCCTG ACACCTCCCAGGCTGGGGCTT	no
J	TCTCCCCCCAGGTTTTTGTGAGG TCTCCCCCCAGGTAATGTAAGTG	yes	ACACCTCCCAGGCTTAGCCTG ACACCTCCCA-----------GCTTGCAGGA ACACCACTAAAATGGGGC	no
K	TCTCCCCCCAGGTTTTTGTGAGG TCTCCCCCCAGGTGAGGGAAGA	yes	ACACCTCCCAGGCTTTTGTGG ACACCTCCCACACATCGCCTG	yes
L	TCTCCCCCCAGGTTTTTGTGAGG TCTCCCCCCAGGTTTTTTGTGAAG	yes	ACACCTCCCAGGCTTTTGTGG ACACCTCCCAGGCTTCCAGGA	yes
M	TCTCCCCCCAGGTTTTTGTGAGG	yes	ACACCTCCCAGGCTTTTGTGG ACACCTCCCAGGCTTGCAGGG	yes
N	TCTCCCCCCAGGTTTTTGTGAGG TCTCCCCCCAGGTTTTGGGAAGGG	yes	ACACCTCCCAGGCTTTTGTGG	yes

ISSN: 1472-6750