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Table 1 summary of PCR information

From: Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5

Fragment Primers code Primers sequence Template Length of PCR product (bp) restriction enzyme
ES 1411Sh5EF1aF1 ccggtgtaca caggaagtga caat pShuttle 181 BsrGI
1411Sh5EF1aR1 cttttgtatg aattactcga cgtcagtatt acgcgctatg agtaacacaa AatII
EF1ap 1411Sh5EF1aF2 cgcgtaatac tgacgtcgag taattcatac aaaaggactc gc pLVX-EF1a-Tet3G 1360 AatII
1411Sh5EF1aR2 acggtacctc acgacacctg aaatggaaga a KpnI
MCS 1411Sh5EF1aF3 ttccatttca ggtgtcgtga ggtaccgtcg acgcggccgc acgcgttcta self-anneal 80 KpnI
1411Sh5EF1aR3 ggccgatatc ttagctagca agcttaggtc tagaacgcgt gcggccgcgt EcoRV
ES-EF1ap-MCS   overlap extension PCR   1558  
GFP 1703GFP-kf ggccggtacc atggtgagca agggcgagga g pLEGFP-C1 748 KpnI
1703GFP-hr ggccaagctt tagagtccgg acttgtacag ctcgt HindIII
XbaI-HIRGD 1702F11pRGD1 ccagcacgac tgcctatcct tt pFiber5-11p 164 XbaI
1702F11pHIRGD2 gaaacagtct ccgcggcagt cacaatttat tgctcttcgg ttaagcatg  
HIRGD-MfeI 1702F11pHIRGD3 tgtgactgcc gcggagactg tttctgcgac gagacatcat attgtattcg tataac pFiber5-11p 240  
1702F11pRGD4 ctgaatgaaa aatgacttga aattttct MfeI
XbaI-HIRGD-MfeI   overlap extension PCR   380  
XbaI-CRGD 1702F11pRGD1 ccagcacgac tgcctatcct tt pFiber5-11p 284 XbaI
1702F11pCRGD2 tgaaccgcca ccacctgagt cgtcttctct gatgtagtaa aaggta  
CRGD 1702F11pCRGD3 gaagacgact caggtggtgg cggttcaggc ggaggtggct ctggcggtgg cggat self-anneal 90  
1702F11pCRGD4 ggctcagcag aaacagtctc cgcggcagtc acacgatccg ccaccgccag agcca  
CRGD-MfeI 1702F11pCRGD5 cgcggagact gtttctgctg agcccaagaa taaagaatcg pFiber5-11p 105  
1702F11pRGD4 ctgaatgaaa aatgacttga aattttct MfeI
XbaI-CRGD-MfeI   overlap extension PCR   428