Table 1 summary of PCR information
From: Efficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5
Fragment | Primers code | Primers sequence | Template | Length of PCR product (bp) | restriction enzyme |
|---|---|---|---|---|---|
ES | 1411Sh5EF1aF1 | ccggtgtaca caggaagtga caat | pShuttle | 181 | BsrGI |
1411Sh5EF1aR1 | cttttgtatg aattactcga cgtcagtatt acgcgctatg agtaacacaa | AatII | |||
EF1ap | 1411Sh5EF1aF2 | cgcgtaatac tgacgtcgag taattcatac aaaaggactc gc | pLVX-EF1a-Tet3G | 1360 | AatII |
1411Sh5EF1aR2 | acggtacctc acgacacctg aaatggaaga a | KpnI | |||
MCS | 1411Sh5EF1aF3 | ttccatttca ggtgtcgtga ggtaccgtcg acgcggccgc acgcgttcta | self-anneal | 80 | KpnI |
1411Sh5EF1aR3 | ggccgatatc ttagctagca agcttaggtc tagaacgcgt gcggccgcgt | EcoRV | |||
ES-EF1ap-MCS | Â | overlap extension PCR | Â | 1558 | Â |
GFP | 1703GFP-kf | ggccggtacc atggtgagca agggcgagga g | pLEGFP-C1 | 748 | KpnI |
1703GFP-hr | ggccaagctt tagagtccgg acttgtacag ctcgt | HindIII | |||
XbaI-HIRGD | 1702F11pRGD1 | ccagcacgac tgcctatcct tt | pFiber5-11p | 164 | XbaI |
1702F11pHIRGD2 | gaaacagtct ccgcggcagt cacaatttat tgctcttcgg ttaagcatg | Â | |||
HIRGD-MfeI | 1702F11pHIRGD3 | tgtgactgcc gcggagactg tttctgcgac gagacatcat attgtattcg tataac | pFiber5-11p | 240 | Â |
1702F11pRGD4 | ctgaatgaaa aatgacttga aattttct | MfeI | |||
XbaI-HIRGD-MfeI | Â | overlap extension PCR | Â | 380 | Â |
XbaI-CRGD | 1702F11pRGD1 | ccagcacgac tgcctatcct tt | pFiber5-11p | 284 | XbaI |
1702F11pCRGD2 | tgaaccgcca ccacctgagt cgtcttctct gatgtagtaa aaggta | Â | |||
CRGD | 1702F11pCRGD3 | gaagacgact caggtggtgg cggttcaggc ggaggtggct ctggcggtgg cggat | self-anneal | 90 | Â |
1702F11pCRGD4 | ggctcagcag aaacagtctc cgcggcagtc acacgatccg ccaccgccag agcca | Â | |||
CRGD-MfeI | 1702F11pCRGD5 | cgcggagact gtttctgctg agcccaagaa taaagaatcg | pFiber5-11p | 105 | Â |
1702F11pRGD4 | ctgaatgaaa aatgacttga aattttct | MfeI | |||
XbaI-CRGD-MfeI | Â | overlap extension PCR | Â | 428 | Â |