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Table 1 Strains and plasmids used in this study

From: Characterizing active transportation mechanisms for free fatty acids and antibiotics in Synechocystis sp. PCC 6803

  Description Parent Curtiss Collection Name Reference
Strain Name
 SD100 Single colony isolate of wild-type Synechocystis sp. PCC 6803 N/A SD100 30
 SD277 High free fatty acid producing strain SD100 SD277 8
 SD100 slr2131 SD100 with slr2131 deleted SD100 SD659 this research
 SD277 slr2131 SD277 with slr2131 deleted SD100 SD660 this research
 SD100 sll0180 SD100 with sll0180 deleted SD277 SD661 this research
 SD277 sll0180 SD277 with sll0180 deleted SD277 SD662 this research
 SD100 slr2131 pacrB SD100 with slr2131 deleted with expression vector expressing E. coli acrB SD100 slr2131 SD665 this research
 SD277 slr2131 pacrB SD277 with slr2131 deleted with expression vector expressing E. coli acrB SD277 slr2131 SD666 this research
 SD100 sll0180 pSacrA SD100 with sll0180 deleted with expression vector expressing E. coli acrA SD100 sll0180 SD667 this research
 SD277 sll0180 pSacrA SD277 with sll0180 deleted with expression vector expressing E. coli acrA SD277 sll0180 SD668 this research
 SD100 pacrB SD100 p⍦691 (Ec acrB) SD100 SD643 this research
 SD277 pacrB SD277 p⍦691 (Ec acrB) SD277 SD644 this research
 SD100 pacrA SD100 p⍦692 (Ec acrA) SD100 SD645 this research
 SD277 pacrA SD277 p⍦692 (Ec acrA) SD277 SD646 this research
 SD100 pSpec SD100 with empty expression vector conferring Spc resistance SD100 SD703 this research
 SD277 pSpec SD277 with empty expression vector conferring Spc resistance SD277 SD704 this research
 SD100 pKan SD100 with empty expression vector conferring Kan resistance SD100 SD705 this research
 SD277 pKan SD277 with empty expression vector conferring Kan resistance SD277 SD706 this research
Plasmid Name
 pGEM-3Z commercially available cloning vector N/A N/A N/A
 p⍦694 vector pGEM-3Z harboring slr2131 flanking regions with sacB-KanR cassette inserted between pGEM-3Z p⍦694 this research
 p⍦695 vector pGEM-3Z harboring sll0180 flanking regions with sacB-KanR cassette inserted between pGEM-3Z p⍦695 this research
 pSpec Expression vector conferring resistance to streptomycin and spectinomycin RSF1010 p⍦568 this research
 pKan Expression vector conferring resistance to kanamycin RSF1010 p⍦687 this research
 pacrB Expression vector expressing E. coli acrB pSpec p⍦691 this research
 pSacrA Expression vector expressing E. coli acrA pSpec p⍦701 this research
 pacrA Expression vector expressing E. coli acrA pKan p⍦692 this research