Table 1 Strains and plasmids used in this study
Description | Parent | Curtiss Collection Name | Reference | |
|---|---|---|---|---|
Strain Name | ||||
SD100 | Single colony isolate of wild-type Synechocystis sp. PCC 6803 | N/A | SD100 | 30 |
SD277 | High free fatty acid producing strain | SD100 | SD277 | 8 |
SD100 △slr2131 | SD100 with slr2131 deleted | SD100 | SD659 | this research |
SD277 △slr2131 | SD277 with slr2131 deleted | SD100 | SD660 | this research |
SD100 △sll0180 | SD100 with sll0180 deleted | SD277 | SD661 | this research |
SD277 △sll0180 | SD277 with sll0180 deleted | SD277 | SD662 | this research |
SD100 △slr2131 pacrB | SD100 with slr2131 deleted with expression vector expressing E. coli acrB | SD100 △slr2131 | SD665 | this research |
SD277 △slr2131 pacrB | SD277 with slr2131 deleted with expression vector expressing E. coli acrB | SD277 △slr2131 | SD666 | this research |
SD100 △sll0180 pSacrA | SD100 with sll0180 deleted with expression vector expressing E. coli acrA | SD100 △sll0180 | SD667 | this research |
SD277 △sll0180 pSacrA | SD277 with sll0180 deleted with expression vector expressing E. coli acrA | SD277 △sll0180 | SD668 | this research |
SD100 pacrB | SD100 p⍦691 (Ec acrB) | SD100 | SD643 | this research |
SD277 pacrB | SD277 p⍦691 (Ec acrB) | SD277 | SD644 | this research |
SD100 pacrA | SD100 p⍦692 (Ec acrA) | SD100 | SD645 | this research |
SD277 pacrA | SD277 p⍦692 (Ec acrA) | SD277 | SD646 | this research |
SD100 pSpec | SD100 with empty expression vector conferring Spc resistance | SD100 | SD703 | this research |
SD277 pSpec | SD277 with empty expression vector conferring Spc resistance | SD277 | SD704 | this research |
SD100 pKan | SD100 with empty expression vector conferring Kan resistance | SD100 | SD705 | this research |
SD277 pKan | SD277 with empty expression vector conferring Kan resistance | SD277 | SD706 | this research |
Plasmid Name | ||||
pGEM-3Z | commercially available cloning vector | N/A | N/A | N/A |
p⍦694 | vector pGEM-3Z harboring slr2131 flanking regions with sacB-KanR cassette inserted between | pGEM-3Z | p⍦694 | this research |
p⍦695 | vector pGEM-3Z harboring sll0180 flanking regions with sacB-KanR cassette inserted between | pGEM-3Z | p⍦695 | this research |
pSpec | Expression vector conferring resistance to streptomycin and spectinomycin | RSF1010 | p⍦568 | this research |
pKan | Expression vector conferring resistance to kanamycin | RSF1010 | p⍦687 | this research |
pacrB | Expression vector expressing E. coli acrB | pSpec | p⍦691 | this research |
pSacrA | Expression vector expressing E. coli acrA | pSpec | p⍦701 | this research |
pacrA | Expression vector expressing E. coli acrA | pKan | p⍦692 | this research |