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Fig. 3 | BMC Biotechnology

Fig. 3

From: Fast gene disruption in Trichoderma reesei using in vitro assembled Cas9/gRNA complex

Fig. 3

cbh1 disruption in T. reesei by direct transformation of Cas9/gRNA complex and a plasmid containing the pyr4 selection marker. A: SDS-PAGE analysis of the fermentation supernatants of the transformants. M: protein molecular mass marker, lanes 1–2: TU-6; lanes 3–11: transformants (T1, T2, T3, T4, T6, T7, T8, and T9, respectively) that did not express CBH1; B: agarose gel electrophoresis of the PCR products amplifying the cbh1 locus from the transformants that did not express CBH1. M: DNA molecular mass marker; lane 1: TU-6; lane 2–6: the transformants T1, T2, T7, T8, and T9, respectively; C: Schematic diagram showing the inserted DNA fragments in the edited cbh1 locus; D: Characteristics of inserted or deleted DNA fragments. The numbers for the T. reesei genes were counted from the start codon

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