Table 1 Sequences of primers used for real-time PCR analysis
Name | Forward primer (5’-3’) | Reverse primer (5’-3’) |
|---|---|---|
Chondrogenic markers | ||
 Sox9 | TGGCAAGGCTGACCTGAAG | GCTCAGCTCGCCGATGT |
 Col2a1 | TCCTGGCCTCGTGGGT | GGGATCCGGGAGAGCCA |
 Aggrecan | GCCACTGTTACCGCCACTT | CACTGGCTCTCTGCATCCA |
 COMP(cartilage oligomeric matrix protein) | ACTGCCTGCGTTCTAGTGC | CGCCGCATTAGTCTCCTGAA |
Osteogenic markers | ||
 ALP | CCCTTCACTGCCATCCTGTAC | CCATGGAGACGTTCTCTCTCTCA |
 BSP (bone sialoprotein) | ATGCCTGCCTTGTACCACGAGC | TCCATCGAAGAATCAAAGCAGAG |
 Col1a1 | CCGTGCCCTGCCAGATC | CAGTTCTTGATTTCGTCGCAGATC |
 OCN(Osteocalcin) | GGCAGGGAAGTCAGGGTAG | CCCGTGGTTTCCTGGTC |
 OPN(Osteopontin) | GTACCCTGATGCTACAGACG | TTCATAACTGTCCTTCCCAC |
Mature markers | ||
 CMP(cartilage matrix protein) | AAGAACGACGACCAAAAGGAC | CATCCCCTATACCATCGCCA |
 Col10a1 | GGCCCGGCAGGTCATC | TGGGATGCCTTTTGGTCCTT |
 MMP-13 | AGTTTGGCCATTCCTTAGGTCTTG | GGCTTTTGCCAGTGTAGGTATAGAT |
 VEGF | GGCTGGCAACATAACAGAGAA | CCCCACATCTATACACACCTCC |
 β-actin | CTGAACCCTAAGGCCAACCG | GTCACGCACGATTTCCCTCTC |