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Fig. 3 | BMC Biotechnology

Fig. 3

From: Expression of Rift Valley fever virus N-protein in Nicotiana benthamiana for use as a diagnostic antigen

Fig. 3

Purification of the N-protein using nickel affinity chromatography. a Chromatographic trace showing 6xHis-tag N-protein elution (orange line) from the 6xHis-tag-chelating affinity column with increasing imidazole concentration (blue line). b Western blot analysis (top) and Coomassie-stained gel (bottom) of collected fractions. Lane 1 contained PageRuler ™ Prestained protein ladder (Thermo Scientific, MA, USA), C contains crude plant extract, F3 represents unbound wash fraction 3, F10 contained a wash fraction 10, F12–16 protein peak visualised in fractions 12–16. The protein was detected with 1:5000 anti-N primary antibody and 1:5000 anti-rabbit secondary antibody. The purple arrow indicates the pentamer, the green arrow indicates the dimer and the yellow arrow indicates the monomer

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