|
Number of saccharifications
|
Glucose extraction (%)
|
Cellobiose extraction (%)
|
Total sugar
|
|---|
|
1st
|
2nd
|
3rd
|
4th
|
Total
|
1st
|
2nd
|
3rd
|
4th
|
Total
|
|---|
|
4
|
41.6
|
21.6
|
10.0
|
3.5
|
73.2
|
6.0
|
1.1
|
0
|
0
|
7.1
|
83.8
|
|
2
|
26.9
|
44.1
|
–
|
–
|
71.0
|
6.6
|
3.4
|
–
|
–
|
10.0
|
81.0
|
|
2 (recycle)
|
–
|
80.0
|
–
|
–
|
80.0
|
–
|
0
|
–
|
–
|
0
|
80.0
|
- Jet-milled slurry was subjected to two-stage bead milling at 50 °C using 0.5- and 0.1-mmΦ zirconia beads, respectively. An enzyme cocktail of 0.2 mL/g wood biomass (30 FPU/g glucan) was added in each saccharification stage (see Experimental Methods). Phosphate buffer (100 mM) was used to maintain pH 6. All values are the average of two analysis runs
