Simultaneous enzymatic saccharification and comminution for the valorization of lignocellulosic biomass toward natural products

Table 2 Percentage extraction of sugars from SESC-treated cedar slurry with different numbers of saccharification stages (four and two) with and without supernatant recycling

Number of saccharifications	Glucose extraction (%)					Cellobiose extraction (%)					Total sugar
Number of saccharifications	1st	2nd	3rd	4th	Total	1st	2nd	3rd	4th	Total	Total sugar
4	41.6	21.6	10.0	3.5	73.2	6.0	1.1	0	0	7.1	83.8
2	26.9	44.1	–	–	71.0	6.6	3.4	–	–	10.0	81.0
2 (recycle)	–	80.0	–	–	80.0	–	0	–	–	0	80.0

Jet-milled slurry was subjected to two-stage bead milling at 50 °C using 0.5- and 0.1-mmΦ zirconia beads, respectively. An enzyme cocktail of 0.2 mL/g wood biomass (30 FPU/g glucan) was added in each saccharification stage (see Experimental Methods). Phosphate buffer (100 mM) was used to maintain pH 6. All values are the average of two analysis runs