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Table 2 Percentage extraction of sugars from SESC-treated cedar slurry with different numbers of saccharification stages (four and two) with and without supernatant recycling

From: Simultaneous enzymatic saccharification and comminution for the valorization of lignocellulosic biomass toward natural products

Number of saccharifications Glucose extraction (%) Cellobiose extraction (%) Total sugar
1st 2nd 3rd 4th Total 1st 2nd 3rd 4th Total
4 41.6 21.6 10.0 3.5 73.2 6.0 1.1 0 0 7.1 83.8
2 26.9 44.1 71.0 6.6 3.4 10.0 81.0
2 (recycle) 80.0 80.0 0 0 80.0
  1. Jet-milled slurry was subjected to two-stage bead milling at 50 °C using 0.5- and 0.1-mmΦ zirconia beads, respectively. An enzyme cocktail of 0.2 mL/g wood biomass (30 FPU/g glucan) was added in each saccharification stage (see Experimental Methods). Phosphate buffer (100 mM) was used to maintain pH 6. All values are the average of two analysis runs