Fig. 1

Fluorescence microscopy of desmin-positive rat primary myoblasts. Myoblasts were enriched with a preplate-technique by seeding the supernatant of isolated cells into new flasks after two, 24, and 48 h. The third preplate was further passaged until passage 3. Merge of DAPI (blue) and desmin (red, with Alexa Fluor 647 as secondary antibody) showed that nearly all cells were myoblasts (desmin-positive). Insert shows fibroblasts isolated from rat skin negative for desmin