Table 7 Kinetic Parameters for A. niger cell growth and pectinase production under different cultivation conditions
Parameters | Shake Flask | Bioreactor | |||
|---|---|---|---|---|---|
Un-optimized Medium | Optimized Medium | Batch | Fed-batch | ||
Controlled pH | Uncontrolled pH | ||||
Xmax (g/L) | 2.26 | 2.29 | 3.28 | 2.38 | 6.58 |
μ (h−1) | 0.01 | 0.01 | 0.01 | 0.01 | n.d. |
Pecmax (U/mL) | 27.2 (60Â h) | 76.35 (36Â h) | 109.63 (84Â h) | 99.55 (84Â h) | 450 (108Â h) |
QPec (U/mL/h) | 0.57 | 2.12 | 0.86 | 0.94 | 7.33 |
YPec/X (U/g) | 12,517 (48Â h) | 38,270 (36Â h) | 46,283 (42Â h) | 43,760 (84Â h) | 75,762 (94Â h) |