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Table 5 BB design of the experimental setup for response surface design with experimental values of total enzyme and cell biomass

From: Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system

Run Pectin (g/L) (NH4)2SO4 (g/L) K2HPO4 (g/L) Response pectinase (U/mL) Response CDW (g/L)
1 50.00 3.50 0.50 30.00 1.75
2 50.00 6.00 1.25 39.00 2.00
3 50.00 3.50 0.50 32.00 2.00
4 10.00 6.00 1.25 71.55 1.30
5 30.00 6.00 2.00 83.29 1.55
6 50.00 1.00 1.25 29.00 1.95
7 10.00 1.00 1.25 50.61 1.20
8 30.00 3.50 1.25 101.06 1.95
9 50.00 3.50 2.00 26.00 2.10
10 30.00 3.50 1.25 97.41 1.65
11 10.00 1.00 1.25 50.45 1.30
12 10.00 6.00 1.25 70.28 1.10
13 50.00 6.00 1.25 40.00 2.75
14 30.00 6.00 0.50 66.95 1.30
15 10.00 3.50 0.50 62.99 1.15
16 10.00 3.50 2.00 61.56 1.15
17 50.00 3.50 2.00 23.00 1.90
18 30.00 6.00 2.00 79.96 1.50
19 10.00 3.50 0.50 60.92 1.05
20 30.00 1.00 2.00 85.04 1.40
21 30.00 3.50 1.25 98.05 1.60
22 30.00 3.50 1.25 96.62 1.65
23 30.00 1.00 0.50 59.18 1.85
24 30.00 3.50 1.25 86.62 1.65
25 30.00 3.50 1.25 84.88 1.70
26 50.00 1.00 1.25 25.00 1.65
27 30.00 1.00 0.50 64.57 1.75
28 30.00 1.00 2.00 81.86 1.65
29 30.00 6.00 0.50 63.62 1.55
30 10.00 3.50 2.00 61.87 1.25