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Table 5 BB design of the experimental setup for response surface design with experimental values of total enzyme and cell biomass

From: Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system

Run

Pectin (g/L)

(NH4)2SO4 (g/L)

K2HPO4 (g/L)

Response pectinase (U/mL)

Response CDW (g/L)

1

50.00

3.50

0.50

30.00

1.75

2

50.00

6.00

1.25

39.00

2.00

3

50.00

3.50

0.50

32.00

2.00

4

10.00

6.00

1.25

71.55

1.30

5

30.00

6.00

2.00

83.29

1.55

6

50.00

1.00

1.25

29.00

1.95

7

10.00

1.00

1.25

50.61

1.20

8

30.00

3.50

1.25

101.06

1.95

9

50.00

3.50

2.00

26.00

2.10

10

30.00

3.50

1.25

97.41

1.65

11

10.00

1.00

1.25

50.45

1.30

12

10.00

6.00

1.25

70.28

1.10

13

50.00

6.00

1.25

40.00

2.75

14

30.00

6.00

0.50

66.95

1.30

15

10.00

3.50

0.50

62.99

1.15

16

10.00

3.50

2.00

61.56

1.15

17

50.00

3.50

2.00

23.00

1.90

18

30.00

6.00

2.00

79.96

1.50

19

10.00

3.50

0.50

60.92

1.05

20

30.00

1.00

2.00

85.04

1.40

21

30.00

3.50

1.25

98.05

1.60

22

30.00

3.50

1.25

96.62

1.65

23

30.00

1.00

0.50

59.18

1.85

24

30.00

3.50

1.25

86.62

1.65

25

30.00

3.50

1.25

84.88

1.70

26

50.00

1.00

1.25

25.00

1.65

27

30.00

1.00

0.50

64.57

1.75

28

30.00

1.00

2.00

81.86

1.65

29

30.00

6.00

0.50

63.62

1.55

30

10.00

3.50

2.00

61.87

1.25