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Table 3 PBED with four variables and the actual responses of total enzyme and cell biomass

From: Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system

Run Pectin (g/L) (NH4)2SO4 (g/L) K2HPO4 (g/L) MgSO4.5H2O (g/L) Response pectinase (U/mL) Response CDW (g/L)
1 30.00 1.30 0.50 0.05 65.52 2.20
2 30.00 3.30 0.50 0.50 87.73 2.08
3 10.00 1.30 1.00 0.50 23.00 1.70
4 30.00 3.30 1.00 0.05 52.32 1.80
5 30.00 3.30 0.50 0.50 63.22 1.88
6 30.00 3.30 1.00 0.05 8.94 1.75
7 30.00 1.30 0.50 0.05 51.88 2.33
8 10.00 3.30 1.00 0.50 38.87 1.75
9 10.00 3.30 0.50 0.05 52.99 1.00
10 30.00 1.30 0.50 0.05 58.70 2.45
11 30.00 1.30 1.00 0.05 32.92 1.89
12 30.00 1.30 1.00 0.05 34.90 2.25
13 10.00 3.30 1.00 0.05 33.79 1.00
14 30.00 1.30 1.00 0.50 33.95 3.10
15 10.00 1.30 1.00 0.50 23.00 2.00
16 30.00 1.30 1.00 0.50 34.74 3.05
17 10.00 3.30 1.00 0.05 38.47 1.18
18 30.00 3.30 0.50 0.50 71.71 1.65
19 30.00 1.30 1.00 0.50 33.16 2.65
20 30.00 3.30 0.50 0.50 54.74 2.10
21 10.00 1.30 0.50 0.50 39.66 1.65
22 10.00 1.30 0.50 0.50 42.92 1.75
23 10.00 1.30 0.50 0.05 36.25 1.40
24 10.00 3.30 0.50 0.05 54.89 0.90
25 10.00 3.30 1.00 0.50 38.71 1.65
26 10.00 3.30 1.00 0.50 38.79 1.70
27 30.00 3.30 0.50 0.50 88.85 1.85
28 10.00 3.30 1.00 0.05 43.15 1.25
29 30.00 3.30 0.50 0.50 88.29 2.30
30 10.00 3.30 0.50 0.05 53.94 0.95
31 10.00 1.30 1.00 0.50 23.00 1.85
32 30.00 3.30 1.00 0.05 45.69 1.70
33 10.00 1.30 0.50 0.50 46.17 1.70
34 10.00 1.30 0.50 0.05 42.04 1.30
35 10.00 1.30 0.50 0.05 30.46 1.35
36 30.00 1.30 1.00 0.05 30.94 2.07