Table 3 PBED with four variables and the actual responses of total enzyme and cell biomass
Run | Pectin (g/L) | (NH4)2SO4 (g/L) | K2HPO4 (g/L) | MgSO4.5H2O (g/L) | Response pectinase (U/mL) | Response CDW (g/L) |
|---|---|---|---|---|---|---|
1 | 30.00 | 1.30 | 0.50 | 0.05 | 65.52 | 2.20 |
2 | 30.00 | 3.30 | 0.50 | 0.50 | 87.73 | 2.08 |
3 | 10.00 | 1.30 | 1.00 | 0.50 | 23.00 | 1.70 |
4 | 30.00 | 3.30 | 1.00 | 0.05 | 52.32 | 1.80 |
5 | 30.00 | 3.30 | 0.50 | 0.50 | 63.22 | 1.88 |
6 | 30.00 | 3.30 | 1.00 | 0.05 | 8.94 | 1.75 |
7 | 30.00 | 1.30 | 0.50 | 0.05 | 51.88 | 2.33 |
8 | 10.00 | 3.30 | 1.00 | 0.50 | 38.87 | 1.75 |
9 | 10.00 | 3.30 | 0.50 | 0.05 | 52.99 | 1.00 |
10 | 30.00 | 1.30 | 0.50 | 0.05 | 58.70 | 2.45 |
11 | 30.00 | 1.30 | 1.00 | 0.05 | 32.92 | 1.89 |
12 | 30.00 | 1.30 | 1.00 | 0.05 | 34.90 | 2.25 |
13 | 10.00 | 3.30 | 1.00 | 0.05 | 33.79 | 1.00 |
14 | 30.00 | 1.30 | 1.00 | 0.50 | 33.95 | 3.10 |
15 | 10.00 | 1.30 | 1.00 | 0.50 | 23.00 | 2.00 |
16 | 30.00 | 1.30 | 1.00 | 0.50 | 34.74 | 3.05 |
17 | 10.00 | 3.30 | 1.00 | 0.05 | 38.47 | 1.18 |
18 | 30.00 | 3.30 | 0.50 | 0.50 | 71.71 | 1.65 |
19 | 30.00 | 1.30 | 1.00 | 0.50 | 33.16 | 2.65 |
20 | 30.00 | 3.30 | 0.50 | 0.50 | 54.74 | 2.10 |
21 | 10.00 | 1.30 | 0.50 | 0.50 | 39.66 | 1.65 |
22 | 10.00 | 1.30 | 0.50 | 0.50 | 42.92 | 1.75 |
23 | 10.00 | 1.30 | 0.50 | 0.05 | 36.25 | 1.40 |
24 | 10.00 | 3.30 | 0.50 | 0.05 | 54.89 | 0.90 |
25 | 10.00 | 3.30 | 1.00 | 0.50 | 38.71 | 1.65 |
26 | 10.00 | 3.30 | 1.00 | 0.50 | 38.79 | 1.70 |
27 | 30.00 | 3.30 | 0.50 | 0.50 | 88.85 | 1.85 |
28 | 10.00 | 3.30 | 1.00 | 0.05 | 43.15 | 1.25 |
29 | 30.00 | 3.30 | 0.50 | 0.50 | 88.29 | 2.30 |
30 | 10.00 | 3.30 | 0.50 | 0.05 | 53.94 | 0.95 |
31 | 10.00 | 1.30 | 1.00 | 0.50 | 23.00 | 1.85 |
32 | 30.00 | 3.30 | 1.00 | 0.05 | 45.69 | 1.70 |
33 | 10.00 | 1.30 | 0.50 | 0.50 | 46.17 | 1.70 |
34 | 10.00 | 1.30 | 0.50 | 0.05 | 42.04 | 1.30 |
35 | 10.00 | 1.30 | 0.50 | 0.05 | 30.46 | 1.35 |
36 | 30.00 | 1.30 | 1.00 | 0.05 | 30.94 | 2.07 |