Expression systems | AT-HIS expression system | CF expression system | SA-ELK16 expression system |
---|---|---|---|
Required steps | Step 1: Expression of fusion protein CeA-Mxe-PT-His6 | Step 1: Expression of antibacterial peptide His6-CeA | Step 1: Expression of fusion protein CeA-Mxe-ELK16 |
Step 2: Cell disruption and supernatant collection | Step 2: Ni2+ affinity chromatography | Step 2: Cell disruption and acquisition of protein aggregates | |
Step 3: Ni2+ affinity chromatography | Step 3: Intein-mediated cleavage | ||
Step 4: Intein-mediated cleavage | Step 4: acetic acid treatment and collection of CeA peptide. | ||
Step 5: The second Ni2+ affinity chromatography | |||
Time consuming | 5Â days | 2Â days | 2Â days |
Final yield of peptide production (μg/mg wet cell pellet) a | 0.41 | 0.93 b | 6.20 |
Purity of bio-produced Cecropin A peptide (%) | 92.1 | 90.4 | 99.8 |