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Fig. 6 | BMC Biotechnology

Fig. 6

From: Rapid and efficient production of cecropin A antibacterial peptide in Escherichia coli by fusion with a self-aggregating protein

Fig. 6

Combinatorial optimization analysis of Mxe GyrA intein cleavage activity in CeA–Mxe–ELK16 fusion. Different incubation times (a) and concentrations of DTT (40–80 mM) (b) were used to optimize cleavage activity. Different incubation temperatures (4 °C or 25 °C) were also tested (c). P, samples before cleavage with DTT; M, protein marker

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BMC Biotechnology

ISSN: 1472-6750

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