Fig. 1

Schematic diagram of three different fusions and quick purification of CeA–Mxe–ELK16 fusion. a Compositions of the three different fusions (CeA–Mxe–His, CeA–His₆ or His₆–CeA, and CeA–Mxe–ELK16) used in the three different expression systems (AT-HIS system, CF system, and SA-ELK16 system, respectively). “N” and “C” represent the amino and carboxy terminals of the polypeptide; Red arrow indicates the cleavage site of Mxe GyrA intein. b Easy simple purification process for CeA–Mxe–ELK16 fusion