Fig. 4

Deletion of exon 1/intron 1 boundary of Ccnb3 led to abnormal splicing and generated four splicing mutation. a Sequencing peak and sequence of Ccnb3 cDNA of wild type mouse. b, c, d, e. Sequencing peak and sequence of four splicing mutation of Ccnb3 cDNA of Ccnb3-/O mouse. b Splicing mutation 1: 25 bp exon 1 deletion and 31 bp intron 1 retention. c splicing mutation 2: 25 bp exon 1 deletion and 92 bp intron 1 retention. d splicing mutation 3: 25 bp exon 1 deletion and 31 bp intron 1 retention as well as extra 578 bp other nucleotides, 31 bp intron 1 retention was highlighted by yellow color. e splicing mutation 4: 25 bp exon 1 deletion and 92 bp intron 1 retention as well as 578 bp other nucleotides, 92 bp intron 1 retention was highlighted by yellow color