Fig. 2
From: CRISPR/Cas9-mediated genome editing induces gene knockdown by altering the pre-mRNA splicing in mice
Verify the deletion of the boundary of exon 1 and intron 1 of Ccnb3 by DNA sequence. Part of Ccnb3’s exon 1 and intron 1 was amplified by polymerase chain reaction (PCR) and been sequenced. a The sequencing peak of part of Ccnb3’s exon 1 and intron 1 of Ccnb3+/O mouse. b The sequencing peak of part of Ccnb3’s exon 1 and intron 1 of Ccnb3-/O mouse. c The sequence of Ccnb3 exon 1/intron 1 boundary of Ccnb3+/O mouse. d The sequence of Ccnb3 exon 1/intron 1 boundary of Ccnb3-/O mouse. Red bar in a and b show the site that Ccnb3 sequence difference between Ccnb3+/O and Ccnb3-/O is appear. Red character in C is the lacking sequence in Ccnb3-/O. Among them, the underlined shows the deleted part of exon 1sequence of Ccnb3