Fig. 1
From: CRISPR/Cas9-mediated genome editing induces gene knockdown by altering the pre-mRNA splicing in mice
Deletion of the boundary of exon 1 and intron 1 of Ccnb3 by CRISPR/Cas9 system. a guide RNA binding site. sgRNA 1/2 specifically bind the exon 1 of Ccnb3. b The boundary of exon 1 and intron 1 of Ccnb3 (144 bp nucleotides) were deleted by CRISPR/Cas9 system. c Genotyping of mice. Every lane in Fig. 1c was from different mice. Ccnb3 wild type (+): 560 bp; Ccnb3 knockout (−: 144 bp was deleted): 416 bp