Fig. 4

GUS transient assays in the leaves of Nicotiana benthamiana plants. a The plasmids used in the transient assay. Mini35S represents the truncated CaMV35S promoter (− 46 ~ + 10 bp). The test constructs consisted of PD7~ 8-mini35S, PD8~ 9-mini35S and PD7~ 9-mini35S, in which the 162-bp (− 456 ~ − 295 bp), 111-bp (− 294 ~ − 184 bp) and 273-bp (− 456 ~ − 184 bp) fragments identified in the AtSCPL30 promoter were fused to the mini35S promoter to drive GUS expression. b The fused plasmids were confirmed by restriction digestion analysis with BamHI/PstI. M, Molecular marker DL2000; 1, PD7~ 9-mini35S; 2, PD7~ 8-mini35S; 3, PD8~ 9-mini35S. c Histochemical GUS staining resulting from non-transformed leaves (WT) and transiently transformed leaves with constructs mini35S, PD7~ 8-mini35S, PD8~ 9-mini35S and PD7~ 9-mini35S under normal conditions. d GUS activity in the transiently transformed tobacco leaves with constructs mini35S, PD7~ 8-mini35S, PD8~ 9-mini35S and PD7~ 9-mini35S under normal conditions. The results are the mean ± SD from three experiments (n = 23). Different lowercase letters above the bars indicate significant differences at P < 0.05