Fig. 4

Deleted 22 bp nucleotides in exon 4 of locust LmigOr35 result in alternative splicing and stochastic exon 4 skipping. a Genotype of WT and 22 bp mutant (− 22) locusts. The WT locusts obtain a 485 bp brand and the − 22 bp mutants obtain a 463 bp brand. b PCR product sequencing of the WT and − 22 bp mutant locust LmigOr35 shows that 22 bp nucleotide is missed in the exon 4 of − 22 bp mutant. c RT-PCR shows that WT only has a 380 bp brand and − 22 bp mutants have two brands, namely, the 358 bp and 224 bp brands. d RT-PCR product sequencing of the WT and − 22 bp mutant locust LmigOr35. In the WT locust LmigOr35 cDNA, exon 3 normally combines with exon 4. In the − 22 bp mutant locust LmigOr35 cDNA, exon 3 combines with exon 4 (without the − 22 bp nucleotides) in 358 bp brand (− 22 bp − 1), whereas exon 3 combines with exon 5 and exon 4 is skipped in the 224 bp brand (− 22 bp − 2)