Table 1 List of plasmids used in this study
From: A rapid in vitro method to flip back the double-floxed inverted open reading frame in a plasmid
list of plasmids | ||||||
|---|---|---|---|---|---|---|
Plasmid | Name | Size of DIO or SIO (kb) | Gene of interest | Promoter | Enzymes for cloning experiments | Enzyme for in vitro experiments |
#1 | pAAV-Ef1a-DIO-ORF1 | 2.4 | ORF1 | smCBA | PstI, | XhoI, BsrGI, NsiI |
#2 | pAAV-smCBA-DIO-ORF1 | 2.4 | ORF1 | Ef1a | NcoI, AgeI, XhoI | |
#3 | pAAV-EF1a-DIO-EGFP | 0.9 | EGFP | Ef1a | NheI, EcoRI, BamHI, | XhoI, BsrGI |
#4 | pAAV-Ef1a-DIO-tdtomato | 1.5 | tdTomato | Ef1a | BamHI, XhoI, BstXI | |
#5 | pAAV-Ef1a-DIO-ORF2 | 1.7 | ORF2 | Ef1a | BamHI, EcoRV | |
#6 | pAAV-Ef1a-SIO(loxP)-ORF1 | 2.4 | ORF1 | Ef1a | PstI | PstI |
#7 | pAAV-Ef1a-SIO(lox2272)-ORF1 | 2.4 | ORF1 | Ef1a | PstI | PstI |