Fig. 2From: Engineering Geobacillus thermodenitrificans to introduce cellulolytic activity; expression of native and heterologous cellulase genesExpression constructs of two putative endoglucanase encoding genes derived from the G. thermodenitrificans metagenome analysis. Gene GE39 encodes a GH5 catalytic domain and gene GE40 encodes a GH5 catalytic domain followed by an immunoglobulin-like domain (Ig) and a carbohydrate module of family 46 (CBM). Both genes were expressed in E. coli BL21(DE3) using the pCDF-1b plasmid under control of the lac operatorBack to article page