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Table 3 Sensitivity analysis of the bidirectional DNA walking method, including the Cry-F and Cry-R walking direction, using the four different mixes of DRT primers (A-D)

From: Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes

  Cry-F
  2000 HGEs 200 HGEs 20 HGEs
Bt11 maize + + +
A B C D A B C D A B C D
550 500 650
950
1250
450
1000
1400*
1800
550 500   450
1000
1400*
1800
550    1000*
1400
1800
MON810 maize + + +
A B C D A B C D A B C D
550*
850
1300
650   400     400*    1400 400*
T304–40 cotton + + +
A B C D A B C D A B C D
550
800
350 450
500
600
800*
1200
400
600
550*
800
1000
500
1000
450
500
600
800
1200
400
500
1000
1700
250
550*
500   400
Cry-R
Bt11 maize + + +
A B C D A B C D A B C D
400*
800
> 2000 500
1000
1400
550
1300
1600
300
400*
> 2000 1200 300
550
1700
300
400*
  500
1000
550
1000
MON810 maize + + +
A B C D A B C D A B C D
250* 770 350
500
260 250* 600 500 350 250* 350   350
T304–40 cotton + + (+)
A B C D A B C D A B C D
550 200 200
450
550
200
450
700*
250
550
200*
1000
550 250
450
250 200* 550 450
  1. For each tested sample (Bt11, MON810 and T304–40), 2000, 200, 20 HGEs of the target were tested. For each result, the experiment was carried out in triplicate. The detection of the targets is symbolized by + (amplicons observed in each repetition) or (+) (amplicons not observed in each repetition). The approximate size of amplicons is indicated in base-pair for each DRT primers. The amplicons observed in each repetition (3/3) are indicated in bold while the amplicons obtained not in each repetition (1–2/3) are indicated in italic. The sequenced amplicons are indicated by an asterisk