Skip to main content

Table 2 Effects of metal cations and reagents on the recombinant enzyme activity

From: Cloning and characterization of enoate reductase with high β-ionone to dihydro-β-ionone bioconversion productivity

Cation of reagent Relative activity Cation of reagent Relative activity
1 mM 5 mM 1 mM 5 mM
Control 100.0 100.0 Mn2+ 78.3 114.5
Al3+ 117.0 125.9 Hg2+ 2.1 2.0
Fe3+ 101.7 0.7 Ni2+ 112.3 111.5
Ba2+ 103.2 126.5 Na+ 122.51 130.7
Ca2+ 113.4 123.4 K+ 111.7 123.1
Co2+ 85.8 117.9 Li+ 89.9 118.3
Cu2+ 17.0 2.8 NH4+ 110.5 129.2
Mg2+ 108.6 123.0 DTT 126.6 125.3
Zn2+ 106.5 37.1 NADPH 100.0 136.6
Sr2+ 115.2 121.8 No NADPH 0.0 0.0
Fe2+ 118.0 78.6    
  1. Values shown are the mean of duplicate experiments, and the variation about the mean was below 3%