Cloning and characterization of enoate reductase with high β-ionone to dihydro-β-ionone bioconversion productivity

Table 2 Effects of metal cations and reagents on the recombinant enzyme activity

Cation of reagent	Relative activity		Cation of reagent	Relative activity
Cation of reagent	1 mM	5 mM	Cation of reagent	1 mM	5 mM
Control	100.0	100.0	Mn²⁺	78.3	114.5
Al³⁺	117.0	125.9	Hg²⁺	2.1	2.0
Fe³⁺	101.7	0.7	Ni²⁺	112.3	111.5
Ba²⁺	103.2	126.5	Na⁺	122.51	130.7
Ca²⁺	113.4	123.4	K⁺	111.7	123.1
Co²⁺	85.8	117.9	Li⁺	89.9	118.3
Cu²⁺	17.0	2.8	NH₄⁺	110.5	129.2
Mg²⁺	108.6	123.0	DTT	126.6	125.3
Zn²⁺	106.5	37.1	NADPH	100.0	136.6
Sr²⁺	115.2	121.8	No NADPH	0.0	0.0
Fe²⁺	118.0	78.6

Values shown are the mean of duplicate experiments, and the variation about the mean was below 3%

ISSN: 1472-6750