Fig. 7

Stability of albumin fusion proteins in plasma. Panel a: Prothrombin times (PT) were measured for reactions containing 13.3 μM fusion protein in saline vehicle, or saline alone. Test samples were combined with human normal pooled plasma (NHPP) and preincubated at 37 °C prior to addition of calcium and PT reagent containing tissue factor for times indicated on the x axis. The mean of 12 determinations ± SD is shown for reactions containing test proteins or solutions shown in the legend at right, Panel b: As in Panel a, but for varying concentrations of purified HV3HSA preincubated with NHPP prior to PT initiation for two minutes. Data points are the mean of seven determinations ± SEM. Note y axis break. Panel c: As in Panel b, but for reactions supplemented with the proteins or solutions specified below the x axis. ***, p < 0.001 versus Saline by non-parametric ANOVA with Dunn’s post-tests. Panel d: As in Panel c, but mouse pooled plasma was substituted for NHPP. Note y axis break. **, p < 0.01, and ***, p < 0.001 versus Saline by non-parametric ANOVA with Dunn’s post-tests. Note that results of > 400 s mean that no clotting was detected at the end of the observation period