Fig. 2

Electrophoretic and immunological characterization of fusion proteins.Panel a depicts a 10% SDS-polyacrylamide gel electrophoresed under reducing conditions and stained with Coomassie Brilliant Blue (Gel). Lanes contain markers (MW) or pre-stained markers (PS) or 500 ng of purified proteins (identified above the lanes). Recombinant His-tagged Alpha-1 Protease Inhibitor (API) (purified from transformed E. coli Top10 as described [39]), or human plasma-derived α-thrombin B chain (IIa B) served as non-HSA-related or non-HSA, non-His tagged controls. Panels b-d depict immunoblots of replicate gels identical to that shown in Panel a, except that 250 ng of purified proteins were used per lane, and gels were transferred to nitrocellulose and probed with antibodies specific to HSA, the hexahistidine tag, or the myc epitope (Anti-HSA, Anti-H6, or Anti-myc respectively). PS markers were (in kDa): 180; 130; 95; 72; 55; 43; 34; and 26. MW markers were (in kDa): 200; 150; 120; 100; 85; 70; 60; 50 (greater intensity); 40; 30; 25; and 20