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Table 1 Fluorescence ratio of teratmethlrhodamin-labelled dextran in rat embryos

From: Successful production of genome-edited rats by the rGONAD method

Stain Poring Pulse Voltage (V) Transfer pulse
No. of Pulses
No. of > 2-cell embryos (A) No. of embryos with fluorescence (B) % (B/A)
WKY 50 3 138 68 49.3
50 6 116 86 74.1
40 3 158 36 22.8
40 6 124 56 45.2
30 6 123 38 30.9
DA 50 6 118 88 74.6
40 6 109 41 37.6
30 6 110 23 20.9
WKY x DA (F1) 50 6 122 67 54.9
40 6 154 54 35.1
30 6 133 31 23.3