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Table 1 Fluorescence ratio of teratmethlrhodamin-labelled dextran in rat embryos

From: Successful production of genome-edited rats by the rGONAD method

Stain

Poring Pulse Voltage (V)

Transfer pulse

No. of Pulses

No. of > 2-cell embryos (A)

No. of embryos with fluorescence (B)

% (B/A)

WKY

50

3

138

68

49.3

50

6

116

86

74.1

40

3

158

36

22.8

40

6

124

56

45.2

30

6

123

38

30.9

DA

50

6

118

88

74.6

40

6

109

41

37.6

30

6

110

23

20.9

WKY x DA (F1)

50

6

122

67

54.9

40

6

154

54

35.1

30

6

133

31

23.3