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Fig. 3 | BMC Biotechnology

Fig. 3

From: Soluble expression of an amebic cysteine protease in the cytoplasm of Escherichia coli SHuffle Express cells and purification of active enzyme

Fig. 3

Effect of the E-64 concentration on the protease activity of recombinant EhCP1. The enzyme and inhibitor (0–6 μM) interact for 15 min before the assay. Hydrolysis of 200 μM Z-Arg-Arg-pNA (a) and 10 μM Z-Arg-Arg-AMC (b) by recombinant EhCP1 (as in Fig. 2). Data represent the mean ± S.E.M. (bars) of 3 independent experiments

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BMC Biotechnology

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