Fig. 2

a Effect of pH on rBAxn and engineered enzymes activity using 1% beechwood xylan as substrate. All data are average of three individual experiments. b Effect of temperature on rBAxn activity using 1% beechwood xylan as substrate. All data are average of three individual experiments. c Stability of engineered enzymes (Y124A, W187A and W187A_Y124A) and rBAxn at 50 °C and 80 °C using 1% beechwood xylan under standard conditions. All values are expressed as mean ± SEM, based on three individual experiments. d Stability of engineered enzymes (Y124A, W187A and W187A_Y124A) and rBAxn at pH 9.0 using 1% beechwood xylan under standard conditions. All values are expressed as mean ± SEM, based on three individual experiments