Table 1 Strains and plasmids used in this study
From: Genetic engineering of Escherichia coli to improve L-phenylalanine production
Strains and plasmid | Genotype | Source or reference |
|---|---|---|
E. coli W3110 | Wild type W3110 derived mutant L-tyrosineauxotrophic | Our lab |
Xllp01 | p15A::pheA-Thr326Pro p15A::aroF carried by PL promoter kanr | Our lab |
Xllp02 | Xllp01 ΔptsH | This work |
Xllp03 | Xllp02 Pm37-galp Pm37-glk | This work |
Xllp04 | Xllp02 Pm37-galp Pm93-glk | This work |
Xllp05 | Xllp02 Pm93-galp Pm37-glk | This work |
Xllp06 | Xllp02 Pm93-galp Pm93-glk | This work |
Xllp07 | Xllp04 tyrR S493 T | This work |
Xllp08 | Xllp04 tyrR T495I | This work |
Xllp09 | Xllp04 tyrR N499D | This work |
Xllp10 | Xllp04 tyrR A498V | This work |
Xllp11 | Xllp04 tyrR S482 N | This work |
Xllp12 | Xllp08 pBR322:: tktA carried by trc promoter Ampr | This work |
Xllp13 | Xllp08 pBR322:: ppsA carried by trc promoter Ampr | This work |
Xllp14 | Xllp08 pBR322:: pckA carried by trc promoter Ampr | This work |
Xllp15 | Xllp08 pBR322:: talB carried by trc promoter Ampr | This work |
Xllp16 | Xllp08 pBR322:: aroD carried by trc promoter Ampr | This work |
Xllp17 | Xllp08 pBR322:: ydiB carried by trc promoter Ampr | This work |
Xllp18 | Xllp08 pBR322:: aroC carried by trc promoter Ampr | This work |
Xllp19 | Xllp08 pBR322:: aroD carried by BBa_j23109 promoter Ampr | This work |
Xllp20 | Xllp08 pBR322:: aroD carried by BBa_j23105 promoter Ampr | This work |
Xllp21 | Xllp08 pBR322:: aroD carried by BBa_j23106 promoter Ampr | This work |
Xllp22 | Xllp08 pBR322:: aroD carried by BBa_j23101 promoter Ampr | This work |
Xllp23 | Xllp08 pBR322:: aroD carried by BBa_j23100 promoter Ampr | This work |
p-tktA | pBR322::tktA trc promoter Ampr | This work |
p-ppsA | pBR322::ppsaA trc promoter Ampr | This work |
p-talB | pBR322::talB trc promoter Ampr | This work |
p-pckA | pBR322::pckA trc promoter Ampr | This work |
p-aroD | pBR322::aroD trc promoter Ampr | This work |
p-ydiB | pBR322::ydiB trc promoter Ampr | This work |
p-aroC | pBR322::aroC trc promoter Ampr | This work |
p-j23100-arod | pBR322::aroD BBa_j23100 promoter Ampr | This work |
p-j23101-arod | pBR322::aroD BBa_j23101 promoter Ampr | This work |
p-j23106-arod | pBR322::aroD BBa_j23106 promoter Ampr | This work |
p-j23105-arod | pBR322::aroD BBa_j23105 promoter Ampr | This work |
p-j23109-arod | pBR322::aroD BBa_j23109 promoter Ampr | This work |
pKD46 | applied for gene knocking-out | [18] |
pDS132 | applied for gene knocking-out | [19] |
P15A | applied for gene overexpression | [26] |