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Table 1 Strains and plasmids used in this study

From: Genetic engineering of Escherichia coli to improve L-phenylalanine production

Strains and plasmid Genotype Source or reference
E. coli W3110 Wild type
W3110 derived mutant L-tyrosineauxotrophic
Our lab
Xllp01 p15A::pheA-Thr326Pro p15A::aroF carried by PL promoter kanr Our lab
Xllp02 Xllp01 ΔptsH This work
Xllp03 Xllp02 Pm37-galp Pm37-glk This work
Xllp04 Xllp02 Pm37-galp Pm93-glk This work
Xllp05 Xllp02 Pm93-galp Pm37-glk This work
Xllp06 Xllp02 Pm93-galp Pm93-glk This work
Xllp07 Xllp04 tyrR S493 T This work
Xllp08 Xllp04 tyrR T495I This work
Xllp09 Xllp04 tyrR N499D This work
Xllp10 Xllp04 tyrR A498V This work
Xllp11 Xllp04 tyrR S482 N This work
Xllp12 Xllp08 pBR322:: tktA carried by trc promoter Ampr This work
Xllp13 Xllp08 pBR322:: ppsA carried by trc promoter Ampr This work
Xllp14 Xllp08 pBR322:: pckA carried by trc promoter Ampr This work
Xllp15 Xllp08 pBR322:: talB carried by trc promoter Ampr This work
Xllp16 Xllp08 pBR322:: aroD carried by trc promoter Ampr This work
Xllp17 Xllp08 pBR322:: ydiB carried by trc promoter Ampr This work
Xllp18 Xllp08 pBR322:: aroC carried by trc promoter Ampr This work
Xllp19 Xllp08 pBR322:: aroD carried by BBa_j23109 promoter Ampr This work
Xllp20 Xllp08 pBR322:: aroD carried by BBa_j23105 promoter Ampr This work
Xllp21 Xllp08 pBR322:: aroD carried by BBa_j23106 promoter Ampr This work
Xllp22 Xllp08 pBR322:: aroD carried by BBa_j23101 promoter Ampr This work
Xllp23 Xllp08 pBR322:: aroD carried by BBa_j23100 promoter Ampr This work
p-tktA pBR322::tktA trc promoter Ampr This work
p-ppsA pBR322::ppsaA trc promoter Ampr This work
p-talB pBR322::talB trc promoter Ampr This work
p-pckA pBR322::pckA trc promoter Ampr This work
p-aroD pBR322::aroD trc promoter Ampr This work
p-ydiB pBR322::ydiB trc promoter Ampr This work
p-aroC pBR322::aroC trc promoter Ampr This work
p-j23100-arod pBR322::aroD BBa_j23100 promoter Ampr This work
p-j23101-arod pBR322::aroD BBa_j23101 promoter Ampr This work
p-j23106-arod pBR322::aroD BBa_j23106 promoter Ampr This work
p-j23105-arod pBR322::aroD BBa_j23105 promoter Ampr This work
p-j23109-arod pBR322::aroD BBa_j23109 promoter Ampr This work
pKD46 applied for gene knocking-out [18]
pDS132 applied for gene knocking-out [19]
P15A applied for gene overexpression [26]