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Fig. 6 | BMC Biotechnology

Fig. 6

From: Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast and kinetics

Fig. 6

Spontaneous calcium activity of neurons in hippocampus of freely behaving mouse visualized with iYTnC2 and nVista HD system. a Photo of nVista HD miniature microscope head-mounted to the mouse. b Detected calcium spikes and the average one; spikes exceeding 4 MAD threshold were aligned at the moment of the very start of the peak (0 s). c Spatial filters and sample traces obtained from an imaging session with a freely behaving mouse expressing iYTnC2. Stars over traces denote spikes that were counted as 4 MAD threshold crossings. The sensor was delivered to the hippocampus by means of rAAV (AAV-CAG-NES-iYTnC2) particles

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BMC Biotechnology

ISSN: 1472-6750

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