Fig. 4
Temporal stability of Luc activity in Chlamydomonas transformants obtained through electroporation or Agrobacterium-mediated transformation. Twenty Chlamydomonas transformants showing the highest level of luciferase expression were collected from two experiments (experiments A and B: ten transformants chosen for each experiment, see Additional file 7: Table S4) and analyzed for luciferase expression at the 3rd (black bars) and the 20th (gray bars) cycles of subculture after the initial transformation event. a Electroporation with pAgroLucR b Agrobacterium-mediated trasformation with pAgroLucR c Agrobacterium-mediated trasformation with pAgroLucL. Luminescence is expressed as CPS normalized for 105 cells